Anti-CD49e [SNAKA51]

A mouse monoclonal antibody recognising human CD49e/integrin alpha 5, used to study fibronectin receptor biology, cell adhesion and integrin signalling.

Contributors

Inventor Institute
Martin Humphries University of Manchester
SKU: 152487
Product description: Anti-CD49e [SNAKA51] is a mouse monoclonal antibody that recognises human CD49e, also known as integrin alpha 5. It is used to detect and study alpha 5 beta 1 integrin activity, supporting research into fibronectin receptor biology, cell adhesion and integrin-mediated signalling. Reported applications include immunofluorescence, functional assays and western blot.
Alternate name: Integrin Subunit Alpha 5; Fibronectin Receptor Subunit Alpha; CD49 Antigen-Like Family Member E; Integrin Alpha-F; VLA-5; FNRA; Very Late Activation Protein 5; Alpha Subunit; Fibronectin Receptor; Alpha Polypeptide; Fibronectin Receptor; Alpha Subunit; Fibronectin Receptor; Alpha Polypeptide; Integrin Alpha; CD49e Antigen; CD49e; VLA5A
Additional notes: CD49e is the integrin alpha 5 chain. Integrins are heterodimeric membrane proteins composed of alpha and beta chains; integrin alpha 5 commonly pairs with integrin beta 1 to form the alpha 5 beta 1 fibronectin receptor.
Conjugation: Unconjugated
Isotype: IgG2a kappa
Immunogen: Whole cells corresponding to human Integrin alpha5.
Myeloma used: P3X63Ag8.653
Cat. #: 152487
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Apoptosis and autophagy;Cell biology;Cell signaling and signal transduction;Immunology
Application: IF ; Fn ; WB
Target: Integrin alpha5 (CD49e)
Reactivity: Human
Clone: SNAKA51
Host: Mouse
Class: Monoclonal
Target background: CD49e is the integrin alpha 5 chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor that is known variously: in lymphocytes as very late (activation) antigen 5 (VLA5); in platelets as glycoprotein IcIIa; and in fibroblasts as extracellular matrix receptor 6 (ECM…
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Valdembri et al. 2009. PLoS Biol. 7(1):e25. PMID: 19175293.

Clark et al. 2005. J Cell Sci. 118(Pt 2):291-300. PMID: 15615773.

Images

View Gallery
Immunofluorescence on endothelial cells transfected with mNrp1-mRFP and Rab5-GFP and then incubated with anti-CD49e [SNAKA51]. mNrp1-mRFP and active a5b1 colocalize into Rab5-GFP-positive early endosomes as shown in merging (arrowheads). Lower panels are magnifications of the boxed areas shown in the upper panels. Taken from Valdembri et al. 2009. PLoS Biol. 7(1)

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