HBRN Mouse

Improved model of abrogation of hepatic P450 function.

Contributors

Inventor Institute
Colin Henderson ; Roland Wolf University of Dundee
SKU: 151773
Product description: Improved model of abrogation of hepatic P450 function. The dual deletion of both Cyb5 and POR almost completely abrogates all hepatic cytochrome P450 activities; the new HBRN model thus provides a more authentic hepatic-P450 null phenotype
Conditional: No
Genetic background: HBRN (Cytb5lox/lox::PORlox/lox CreALB) and wild-type (WT; PORlox/lox::Cyb5lox/lox) were generated by crossing HRN (PORlox/lox + CreALB ) and floxed cytochrome b5 mice (Cytb5lox/lox), and thereafter maintained by crossing of homozygous pairs within each line.
Production details: HBRN (Cytb5lox/lox::PORlox/lox Âą CreALB) and wild-type (WT; PORlox/lox::Cyb5lox/lox) were generated by crossing HRN (PORlox/lox + CreALB ) and floxed cytochrome b5 mice (Cytb5lox/lox), and thereafter maintained by crossing of homozygous pairs within each line.
Cat. #: 151773
Research Fields: Cell signaling and signal transduction;Genetics;Metabolism
Target: P450, Cyb5
Zygosity: HBRN (Cytb5lox/lox::PORlox/lox ą CreALB)
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Henderson et al. 2013. Mol Pharmacol. 83(6):1209-17. PMID: 23530090.

Evidence that cytochrome b5 and cytochrome b5 reductase can act as sole electron donors to the hepatic cytochrome P450 system.

Henderson et al. 2003. J Biol Chem. 278(15):13480-6. PMID: 12566435.

Inactivation of the hepatic cytochrome P450 system by conditional deletion of hepatic cytochrome P450 reductase.

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