C10 Colorectal Cell Line

This C10 colorectal cell line was established from surgically resected colorectal adenocarcinomas. Research Area, Cancer, Drug Discovery & Development.

Contributors

Inventor Institute
Walter Bodmer University of Oxford
Cat. #: 151755
Unit size: 1×10^6 cells / vial
Cancer types: Digestive / Gastrointestinal cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Colon
Model: Tumour line
Cancer Types In Detail: Colorectal;Dukes' stage B;Human colorectal adenocarcinoma;Moderately well differentiated
Product description: The C10 cell line was established from a 71-year old male patient with moderately well differentiated adenocarcinoma of the descending colon classified as Dukes' stage B. C10 harbours a well-characterised TP53 point mutation located in exon 7, codon 245, involving a G-to-A nucleotide substitution that results in a glycine-to-serine amino acid change.
Conditional: Yes
Production details: This colorectal cell line was established from surgically resected colorectal adenocarcinomas.
Cellosaurus ID: CVCL_5245
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2mM Glutamine
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. C10 cells grow very slowly; following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
Temperature: 37° C
Atmosphere: 5% CO2
References:

Bracht et al. 2010. Br J Cancer. 103(3):340-346. PMID: 20606684.

Liu et al. 2006. Proc Natl Acad Sci U S A. 103(4):976-981. PMID: 16418264.

Rowan et al. 2000. Proc Natl Acad Sci U S A. 97(7):3352-3357. PMID: 10737795.

Efstathiou et al. 1999. Proc Natl Acad Sci U S A. 96(5):2316-2321. PMID: 10051639.

Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-2845. PMID: 8464898.

Images

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C10 Cell Line - growth profile. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.

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