The hTERT-RPE1 mRuby-APC15 Cell Line is a tet-inducible APC15 reporter cell line.
| Inventor | Institute |
|---|---|
| Jonathon Pines | University of Cambridge |
| SKU: | 151710 |
|---|---|
| Product description: | The hTERT-RPE1 mRuby-APC15 Cell Line is a tet-inducible APC15 reporter cell line. APC15 is a component of the human cell cycle Anaphase promoting complex/cyclosome (APC/C). It is required for progression from metaphase during cell cycle. The uncharacterised open reading frame C11orf51 has been identified in a systematic proteomic analysis of APC/C purified from HeLa cell extracts. Human C11orf51 is conserved in vertebrates and invertebrates and has homology to Schizosaccharomyces pombe APC15 (PM gene ID: 2540655), and Schizosaccharomyces cerevisiae Mnd2 (PM gene ID: 854843). |
| Conditional: | Yes |
| Conditional description: | Tetracycline inducible |
| Production details: | APC15 was amplified by PCR from a full length cDNA clone (ORFeome clone 100066673). Tetracycline inducible cell lines expressing APC15 IRES2 mRuby were generated using the FLIP in system and a modified pCDNA5/FRT/TO vector. The parental tetracycline inducible RPE cell line was created by random integration of an FRT site and a Tet repressor gene into hTERT RPE1 cells followed by selection of a clone that showed high levels of gene expression repression in the absence of tetracycline. |
| Cellosaurus ID: | CVCL_5F68 |
| Parental cell line: | hTERT-RPE1 |
| Cat. #: | 151710 |
|---|---|
| Provenance and Ownership: | The hTERT-RPE1 mRuby-APC15 Cell Line is owned by Cancer Research UK. CancerTools supplies the hTERT-RPE1 mRuby-APC15 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing hTERT-RPE1 mRuby-APC15 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Cell biology |
| Organism: | Human |
| Tissue: | Eye |
| Model: | Transgenic |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | F12/DMEM (1:1) media supplemented with 10% FCS, sodium bicarbonate, glutamate and penicillin/streptavidin. |
| References: |
Mansfield et al. 2011. Nat Cell Biol. 13:1234-43. PMID: 21926987 |
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