BRaf1 KI Mouse

Conditional knockin of oncogenic B-Raf V600E; in vivo study of oncogenic B-raf mutation and signalling pathways

Contributors

Inventor Institute
Catrin Pritchard University of Leicester
SKU: 151519
Product description: Conditional knockin of oncogenic B-Raf V600E; in vivo study of oncogenic B-raf mutation and signalling pathways The LSL-B-Raf-V600E targeting vector, containing a loxP flanked minigene+polyA encoding wildtype exons 15 to 18, amd a loxP flanked resistance cassette, and a substitution of residue 600 (V to E) in the endogenous exon 15, was transfected into E14.1 ES cells. Properly targeted ES cells containing homologous recombination events were injected into C57BL6 balstocysts. Chimeric mice were backcrossed on the C57BL6 background.
Alternate name: LSL B-RafV6E
Conditional: Yes
Conditional description: B-Raf gene with loxP sites flanking wildtype exons 15 to 18 (plus STOP) inserted before mutant exons 15 (V600E) to 18, enabling conditional Cre-mediate excision of wildtype exons and simultaneous knockin of mutant exons.
Genetic background: E14.1/C57BL6
Cat. #: 151519
Cancer types: Colorectal cancer; Skin cancer
Research Fields: Cancer; Cell signaling and signal transduction; Genetics
Target: BRaf V600E (oncogenic mutant)
Model: Knock-In
Zygosity: Heterozygous
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Mercer et al. 2005. Cancer Res. 65(24):11493-500. PMID: 16357158.

Related Tools for Mouse models

Cat. # Tool Name
151481 DLL1 Floxed Mouse Key Info View Tool
151467 p23+/- Mouse Key Info View Tool
151482 Jagged1 Floxed Mouse Key Info View Tool
151553 Pik3ca RBD mutant Mouse Key Info View Tool
151557 RafTR Mouse Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.