Anti-Integrin alpha 3 [F35 177-1]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-Integrin alpha 3 [F35 177-1]
• Research fields: Cell biology;Cancer
• Clone: F35 177-1
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 14 kDa
• Strain: Balb/c
• Reactivity: Human
• Host: Mouse
• Application: IHC ; IF
• Description: Integrins are heterodimeric cell surface receptors composed of a and b subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin a3 is found on most cell types. Integrin a3 associates with b1 integrin to form integrin a3b1, which is one of the most abundant keratinocyte integrins and binds laminin 5.
• Immunogen: MTSV1-7 HYE - human breast epithelial cells.
• Isotype: IgG1 kappa
• Myeloma used: P3X63Ag8.653

Target Details

• Target: Integrin a3 (CD49c)
• Molecular weight: 14 kDa
• Target background: Integrins are heterodimeric cell surface receptors composed of a and b subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin a3 is found on most cell types. Integrin a3 associates with b1 integrin to form integrin a3b1, which is one of the most abundant keratinocyte integrins and binds laminin 5.

Applications

• Application: IHC ; IF

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Lustosa et al. 2014. ScientificWorldJournal. 2014:102541. PMID: 24737953.
• Expression profiling using a cDNA array and immunohistochemistry for the extracellular matrix genes FN-1, ITGA-3, ITGB-5, MMP-2, and MMP-9 in colorectal carcinoma progression and dissemination.
• McInroy et al. 2011. Exp Cell Res. 317(17):2468-78. PMID: 21821021.
• Plectin regulates invasiveness of SW480 colon carcinoma cells and is targeted to podosome-like adhesions in an isoform-specific manner.
• Gaggioli et al. 2007. Nat Cell Biol. 9(12):1392-400. PMID: 18037882.
• Fibroblast-led collective invasion of carcinoma cells with differing roles for RhoGTPases in leading and following cells.