NBF0LN9-1315BL Cell Line
Ninth generation cell line derived from an ipsilateral brachial lymph node metastasis of an eighth-generation line
Contributors
| Inventor | Institute |
|---|---|
| Nathan E. Reticker-Flynn | Stanford University |
| Cat. #: | 162234 |
|---|---|
| Cancer types: | Melanoma |
| Organism: | Mouse |
| Tissue: | Skin (lymph node metastasis) |
| Gender: | Female |
| Model: | Tumorigenic cell line; syngeneic |
| Morphology: | Mixed morphology that is predominantly spindle-shaped |
| Cancer Types In Detail: | Melanoma |
| Primary citation: | Reticker-Flynn, N.E., et al. (2022). 185(11):1924-1942.e23. PMID: 35525247 |
| Product description: | Ninth generation cell line derived from an ipsilateral brachial lymph node metastasis of an eighth-generation line |
|---|---|
| Gender: | Female |
| CRISPR: | No |
| Production details: | Line was isolated from a brachial lymph node metastasis after roughly one month of growth of a LN8 tumor |
| Parental cell line: | B16-F0 |
| Model description: | A luciferase transgene was integrated into the B16-F0 parental line, but is no loger expressed in LN metastatic progeny. WES suggests the presence of Braf and Pten mutations. |
| disease: | Melanoma |
| Storage conditions: | Liquid Nitrogen |
|---|---|
| Shipping conditions: | Dry Ice |
| Growth medium: | DMEM (high glucose) + 10% FBS + 4mM L-glutamine (or GlutaMAX) + 1% Pen/Strep (optional) |
| Subculture routine: | See product datasheet |
| Temperature: | 37C |
| Atmosphere: | 5% CO2 |
| Storage medium: | DMEM, 50% FBS, 10% DMSO |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Reticker-Flynn, N.E., et al. (2022). 185(11):1924-1942.e23. PMID: 35525247 |
|---|
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