NBF0LN4-521BL Cell Line

Fourth generation cell line derived from an ipsilateral brachial lymph node metastasis of a third-generation line

Contributors

Inventor Institute
Nathan E. Reticker-Flynn Stanford University
Cat. #: 162229
Cancer types: Skin cancer
Organism: Mouse
Tissue: Skin (lymph node metastasis)
Gender: Female
Model: Tumorigenic cell line; syngeneic
Morphology: Mixed morphology that is predominantly spindle-shaped
Product description: Fourth generation cell line derived from an ipsilateral brachial lymph node metastasis of a third-generation line
Gender: Female
CRISPR: No
Production details: Line was isolated from a brachial lymph node metastasis after roughly one month of growth of a LN3 tumor
Parental cell line: B16-F0
Model description: A luciferase transgene was integrated into the B16-F0 parental line, but is no longer expressed in LN metastatic progeny. WES suggests the presence of Braf and Pten mutations.
Disease: Melanoma
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: DMEM (high glucose) + 10% FBS + 4mM L-glutamine (or GlutaMAX) + 1% Pen/Strep (optional)
Subculture routine: See product datasheet
Temperature: 37C
Atmosphere: 5% CO₂
Storage medium: DMEM, 50% FBS, 10% DMSO
Mycoplasma free: Yes

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