Glioblastoma WHO IV NCH354 cell line

Glioblastoma WHO IV cell line, NCH354

Contributors

Institute
Heidelberg University
Cat. #: 162316
Cancer types: Brain
Organism: Human
Tissue: Brain
Morphology: Adherent
Growth properties: Cells grow as adherent monolayer cultures under standard culture conditions at 37°C in a humidified incubator with 5% CO?. Medium is replaced twice per week.
Primary citation: Wirthschaft et al. Int J Cancer. 143(5):1176-1187, PMID: 29582423.
Product description: NCH354 is a patient-derived IDH?wildtype glioblastoma cell line established from a primary WHO grade IV tumour obtained from a 38?year?old female patient. The primary tumour was reported as MGMT promoter positive. Cells grow as adherent monolayer cultures under standard conditions.
Production details: Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro.
Growth medium: DMEM (high glucose) supplemented with 10% fetal calf serum (FCS), 1% GlutaMAX, and 1% penicillin/streptomycin.
Subculture routine: Cells are passaged at sub-confluent densities using 0.05% Trypsin-EDTA. After enzymatic detachment, trypsin is neutralised with serum-containing medium and cells are reseeded into fresh culture vessels. Passage number should be recorded.
Temperature: 37°C
References:

Wirthschaft et al. 2019. Acta Neuropathol. 138(2):275-293. PMID: 31062076.

Related Tools for Cell lines

Cat. # Tool Name
151014 MCF7 AREc32 Cell Line Key Info View Tool
151425 TR146 Cell Line Key Info View Tool
151450 JIM3 Cell Line Key Info View Tool
151479 HCT 116 p300 KO [D10] Cell Line Key Info View Tool
151629 MEF Raf1 FF/FF KI Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.