IDHmut glioma stem-like NCH551b cell line

Glioma stem-like cell line NCH551b expressing mutant isocitrate dehydrogenase (IDH), used to model low-grade gliomas or secondary glioblastomas.

Contributors

Inventor Institute
Christel Herold-Mende Heidelberg University
SKU: 162301
Product description: NCH551b is a patient-derived glioma stem-like cell line with an IDH mutation, derived from the second relapse of an astrocytoma that progressed from WHO grade II to WHO grade IV. Tumour tissue was obtained from a 40 year old male patient-derived during surgical resection. The cell line is maintained under serum-free conditions and grows as free floating neurospheres.
Gender: Male
Production details: Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro.
Cat. #: 162301
Cancer types: Brain cancer
Organism: Human
Tissue: Brain
Gender: Male
Morphology: Grown as free-floating neurospheres under serum-free culture conditions.
Growth properties: Cells grow as free-floating neurospheres under serum-free conditions in non-adherent culture vessels. Cultures are maintained at 37°C in a humidified incubator with 5% CO2.
Primary citation: Dettling et al. 2018. Clin Cancer Res. 24(12):2951-2962. PMID: 29563135.
Storage conditions: FCS-free freezing medium (Synth-a-freeze medium)
Growth medium: DMEM/F-12–based serum-free medium supplemented with BIT, GlutaMAX, EGF, and FGF-2.
Subculture routine: Neurospheres are passaged at high cell density by gentle mechanical dissociation. Accutase may be used if required to obtain single-cell suspensions. Cells are reseeded in fresh serum-free medium.
Temperature: 37°C
References:

Trong et al. 2023. Cells. 12(8).

Cano-Galiano et al. 2021. Neurooncol Advances. 3(1). PMID: 34250481.

Ricklefs et al. 2020. Int J Mol Sci. 21(6).

Trong et al. 2020. Cells. 9(6).

Biedermann et al. 2019. Cancers. 11(12).

Trong et al. 2018. Int J Mol Sci. 19(10):2903. PMID: 30257451.

Dettling et al. 2018. Clin Cancer Res. 24(12):2951-2962. PMID: 29563135.

Bunse et al. 2018. Nat Med. 24(8):1192-1203. PMID: 29988124.

Pusch et al. 2017. Acta Neuropathol. 133(4):629-644. PMID: 28124097.

Kohanbash et al. 2017. J Clin Invest. 127(4):1425-1437. PMID: 28319047.

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IDHmut glioma stem-like NCH551b cell line

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