Nrf3 deficient mouse

The model was created by replacing two exons of the Nfe2l3 gene, comprising sequences coding for the bZIP domain of the transcription factor by neomycin-cytosine deaminase cassette of the targeting vector. 129S6 ES cells were selected by resistance to G418. The targeted cells were injected into C57BL/6J blastocysts. Resultant chimeras were backcrossed for more than 10 generations. Neomycin-cytosine cassette flanked by loxP sites has been removed by breeding to Cre mice (C57BL/6-Tg(Zp3-cre)93Knw/J). Knockout mice have been generated in 129S6, C57BL/6 and FVB backgrounds.

Contributors

Inventor Institute
Volker Blank McGill University
SKU: 162173
Product description: The model was created by replacing two exons of the Nfe2l3 gene, comprising sequences coding for the bZIP domain of the transcription factor by neomycin-cytosine deaminase cassette of the targeting vector. 129S6 ES cells were selected by resistance to G418. The targeted cells were injected into C57BL/6J blastocysts. Resultant chimeras were backcrossed for more than 10 generations. Neomycin-cytosine cassette flanked by loxP sites has been removed by breeding to Cre mice (C57BL/6-Tg(Zp3-cre)93Knw/J). Knockout mice have been generated in 129S6, C57BL/6 and FVB backgrounds.
Production details: The model was created by replacing two exons of the Nfe2l3 gene, comprising sequences coding for the bZIP domain of the transcription factor by neomycin-cytosine deaminase cassette of the targeting vector. 129S6 ES cells were selected by resistance to G418. The targeted cells were injected into C57BL/6J blastocysts. Resultant chimeras were backcrossed for more than 10 generations. Neomycin-cytosine cassette flanked by loxP sites has been removed by breeding to Cre mice (C57BL/6-Tg(Zp3-cre)93Knw/J). Knockout mice have been generated in 129S6, C57BL/6 and FVB backgrounds.
Cat. #: 162173
Research Fields: Cancer
Zygosity: Homozygous
Strain: Knockout mice have been generated in the following backgrounds: 129S6, C57BL/6, FVB
Phenotype: Nfe2l3-/- mice develp normallyand are fertile.
Primary citation: Blank V. et al. 2004. Molecular and Cellular Biology. Apr. v24(8). PMID: 15060151
Shipping conditions: Sperm / dry ice
References:

Blank V. et al. 2004. Molecular and Cellular Biology. Apr. v24(8). PMID: 15060151

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