Anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]

An antibody that detects K123 monoubiquitinated yeast H2B, homologue to human H2B K120, will enable research to discover the fundamental principles of the regulation and function of monoubiquitination of H2B.

Contributors

Inventor Institute
Alfred Nijkerk UbiQ Bio
SKU: 152107
Product description: C-terminus monoubiquitination of histone H2B acts as a signal for downstream processes such as gene regulation, genome packaging, histone modification crosstalk, and DNA repair. How the ubiquitination levels are controlled and how the Ub signal is translated to downstream effects is poorly understood. An antibody that detects K123 monoubiquitinated yeast H2B, homologue to human H2B K120, will enable research to discover the fundamental principles of the regulation and function of monoubiquitination of H2B. The antibody is developed using an ubiquitin-polypeptide antigen based on the amino-acids 115-130 of the yeast H2B protein, linked by a proteolytically stable bond.
Conjugation: Unconjugated
Isotype: IgG1
Molecular weight: 23 kDa
Immunogen: Ub-polypeptide antigen based on amino acids 115 – 130 of yeast H2B
Cat. #: 152107
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Genetics
Application: WB ; ChIP ; ELISA ; WB
Target: Yeast monoubiquityl-histone H2B
Reactivity: Human ; Schizosaccharomyces pombe ; Saccharomyces cerevisiae
Clone: 1B3F12/A9
Host: Mouse
Class: Monoclonal
Target background: C-terminus monoubiquitination of histone H2B acts as a signal for downstream processes such as gene regulation, genome packaging, histone modification crosstalk, and DNA repair. How the ubiquitination levels are controlled and how the Ub signal is translated to downstream effects is poorly understood. An antibody that detects K123 monoubiquitinated yeast H2B, homologue to human H2B K120, will enable research to discover the fundamental principles of the regulation and function of monoubiquitination of H2B. The antibody is developed using an ubiquitin-polypeptide antigen based on the amino-acids 115-130 of the yeast H2B protein, linked by a proteolytically stable bond.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

van Welsem et al. 2018. Nucleic Acids Res. 46(21):11251-11261. PMID: 30203048.

Dot1 promotes H2B ubiquitination by a methyltransferase-independent mechanism.

Vlaming et al. 2016. Elife. 5:. PMID: 27922451.

Direct screening for chromatin status on DNA barcodes in yeast delineates the regulome of H3K79 methylation by Dot1.

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