DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5′-phosphodiester bond, giving rise to DNA with a free 5′ phosphate.
| Inventor | Institute |
|---|---|
| Ian Waddell | Cancer Research UK, Manchester Institute |
| SKU: | 151785 |
|---|---|
| Product description: | DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5′-phosphodiester bond, giving rise to DNA with a free 5′ phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5′- phosphoglycolates on protruding 5′ ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5′-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a ‘clean’ DSB with 5′-phosphate termini that are ready for ligation. Has also 3′-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation. |
| Conjugation: | Unconjugated |
| Molecular weight: | 49 kDa |
| Immunogen: | Recombinant human protein with his tag |
| Cat. #: | 151785 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Cancer;Genetics |
| Application: | WB |
| Target: | Tdp2 |
| Reactivity: | Human |
| Clone: | Tdp2 |
| Host: | Rabbit |
| Class: | Polyclonal |
| Target background: | DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5′-phosphodiester bond, giving rise to DNA with a free 5′ phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5′- phosphoglycolates on protruding 5′ ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5′-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a ‘clean’ DSB with 5′-phosphate termini that are ready for ligation. Has also 3′-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation. |
|---|
| Format: | Liquid |
|---|---|
| Concentration: | 0.9-1.1 mg/ml |
| Storage conditions: | -15° C to -25° C |
| Shipping conditions: | Dry ice |
| References: |
Thomson et al. 2013. Anal Biochem. 436(2):145-50. PMID: 23416181. Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase. |
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