Anti-MDM2 [SMP14]

MDM2 is over-expressed in many tumours. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein.

Contributors

Inventor Institute
David Lane University of Dundee
Cat. #: 151295
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cancer;Cell biology;Genetics
Application: ELISA ; IHC ; IF ; IP ; WB
Target: Murine Double Minute 2 protein (MDM2)
Reactivity: Human
Clone: SMP 14
Host: Mouse
Class: Monoclonal
Product description: MDM2 is over-expressed in many tumours. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein.
Conjugation: Unconjugated
Isotype: Ig3 Kappa
Immunogen: Synthetic peptide CSRPSTSSRRRAISE (residues 154-167 in human MDM2) cross-linked to KLH using glutaldehyde.
Myeloma used: P3X63Ag8.653
Target background: MDM2 is over-expressed in many tumours. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Tao et al. 2013. Cell Res. 23(5):620-34. PMID: 23357851.

Jiang et al. 2013. Nature. 493(7434):689-93. PMID: 23334421.

Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.

Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence.

Das et al. 2007. Int J Surg Pathol. 15(4):335-45. PMID: 17913939.

p53 and mdm2 expression in colorectal carcinoma: a correlative analysis with clinical staging and histological parameters.

Hashemi et al. 2002. Cancer Lett. 180(2):211-21. PMID: 12175554.

A melanoma-predisposing germline CDKN2A mutation with Fn significance for both p16 and p14ARF.

Zhang et al. 2001. J Biol Chem. 276(32):29702-10. PMID: 11359766.

Cyclin a-CDK phosphorylation regulates MDM2 protein interactions.

Bttger et al. 1997. J Mol Biol. 269(5):744-56. PMID: 9223638.

Molecular characterization of the hdm2-p53 interaction.

Dowell et al. 1996. Cytopathology. 7(5):340-51. PMID: 8911758.

Expression of p21waf1/Cip1, MDM2 and p53 in vivo: analysis of cytological preparations.

Picksley et al. 1994. Oncogene. 9(9):2523-9. PMID: 8058315.

Immunochemical analysis of the interaction of p53 with MDM2

–fine mapping of the MDM2 binding site on p53 using synthetic peptides.

Images

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Cell staining with SMP14 hybridoma supernatant of MCF7 treated with Nutlin

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