GalNAc-T1 has also been implicated in carcinogenesis.O-glycans are important biomarkers in cancer. The truncated O-glycans comprising Tn formed by the GalNAc transferases and T formed by further elongation by the core1 synthase (C1GalT1) are widely recognized as pancarcinoma antigens.
| Institute |
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| University of Copenhagen |
| SKU: | 155104 |
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| Product description: | GalNAc-T1 is one of many polypeptide GalNAc-transferases that attach GalNAc to proteins forming the GalNAc?ĂÂ?1-O-Ser/Thr linkage for GalNAc-type O-glycosylation. The GalNAc-transferase isoforms have considerably overlapping functions as well as unique distinct functions. GalNAc-T1 and ÄËĂÂĂÂT2 are the main contributors to O-glycosylation of peptides in most cells and they have distinct functions. Murine knock out studies demonstrate that GalNAc-T1 plays important roles in B-cell differentiation. GalNAc-T1 has also been implicated in carcinogenesis. O-glycans are important biomarkers in cancer. The truncated O-glycans comprising Tn formed by the GalNAc transferases and T formed by further elongation by the core1 synthase (C1GalT1) are widely recognized as pancarcinoma antigens. They are masked by sialic acid or further elongation or branching in normal cells. Validation: 1. Positive reaction (IC/IF) in cells expressing GalNAc-T1 using close isoforms as negative controls e.g. GalNAc-T13. 2. Selective IP of active GalNAc-T1 from total cell extracts. 3. Distinct perinuclear staining in cell lines (ICC/IF) and tissues (IHC, IF) suggestive of Golgi localization. 4. loss of staining (IC/IF) following KO of GalNAc-T1 |
| Alternate name: | UH3, 4D8 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Immunogen: | Catalytically active secreted GalNAc-T1 produced in insect cells. Recombinant protein containing aa. 41-559 (Uniprot isoform-1) |
| Immunogen Uniprot ID: | Q10472 |
| Cat. #: | 155104 |
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| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Biochemistry; Cancer; Cell biology |
| Application: | ELISA ; IHC ; IF ; IP |
| Target: | GalNAc-T1/GALNT1 |
| Reactivity: | Human |
| Host: | Mouse |
| Class: | Monoclonal |
| Target background: | GalNAc-T1 is one of many polypeptide GalNAc-transferases that attach GalNAc to proteins forming the GalNAc1-O-Ser/Thr linkage for GalNAc-type O-glycosylation. The GalNAc-transferase isoforms have considerably overlapping functions as well as unique distinct functions. GalNAc-T1 and T2 are the main contributors to O-glycosylation of peptides in most cells and they have distinct functions. Murine knock out studies demonstrate that GalNAc-T1 plays important roles in B-cell differentiation. GalNAc-T1 has also been implicated in carcinogenesis. O-glycans are important biomarkers in cancer. The truncated O-glycans comprising Tn formed by the GalNAc transferases and T formed by further elongation by the core1 synthase (C1GalT1) are widely recognized as pancarcinoma antigens. They are masked by sialic acid or further elongation or branching in normal cells. Validation: 1. Positive reaction (IC/IF) in cells expressing GalNAc-T1 using close isoforms as negative controls e.g. GalNAc-T13. 2. Selective IP of active GalNAc-T1 from total cell extracts. 3. Distinct perinuclear staining in cell lines (ICC/IF) and tissues (IHC, IF) suggestive of Golgi localization. 4. loss of staining (IC/IF) following KO of GalNAc-T1 |
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| Format: | Liquid |
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| Concentration: | 0.9-1.1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | -15° C to -25° C |
| Shipping conditions: | Dry ice |
| References: |
A validated collection of mouse monoclonal antibodies to human glycosyltransferases functioning in mucin-type O-glycosylation. Exploring Regulation of Protein O-Glycosylation in Isogenic Human HEK293 Cells by Differential O-Glycoproteomics. Deconstruction of O-glycosylation–GalNAc-T isoforms direct distinct subsets of the O-glycoproteome. Initiation of GalNAc-type O-glycosylation in the endoplasmic reticulum promotes cancer cell invasiveness. Control of mucin-type O-glycosylation: a classification of the polypeptide GalNAc-transferase gene family. Probing isoform-specific functions of polypeptide GalNAc-transferases using zinc finger nuclease glycoengineered SimpleCells. Expression of polypeptide GalNAc-transferases in stratified epithelia and squamous cell carcinomas: immunohistological evaluation using monoclonal antibodies to three members of the GalNAc-transferase family. |
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