Xist-TetOP mutant: DeltaC cell line

Deletion of mouse Xist lncRNA to dissect specific RNA modules in the process of X-chromosome inactivation.

Contributors

Inventor Institute
Sim?„ƒo Teixeira da Rocha Instituto de Medicina Molecular Jo?„ƒo Lobo Antunes
Cat. #: 160727
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Genetics;Stem cell biology
Organism: Mouse
Model: Stem Cells
Growth properties: No disruption of Polycomb recruitment to the inactive X-chromosome.
Product description: Deletion of mouse Xist lncRNA to dissect specific RNA modules in the process of X-chromosome inactivation.
Conditional: No
Conditional description: Inducible through addition of doxycycline (1.5 ÎĚ?Ÿg/ml) to the medium
Production details: CRISPR/Cas9 genome editing of the Xist-TetOP XY ESC line
Additional notes: Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Ca…
Parental cell line: Xist-TetOP XY ESCs (Wutz et al., 2002 PMID: 11780141)
Format: Frozen
Shipping conditions: Dry ice
Growth medium: ESCs grow in feeder-free classic ESC medium DMEM media containing 15% fetal bovine serum (FBS), 103 U/ml leukemia inhibitor factor (LIF), 10-4 mM 2-mercaptoethanol, 50 U/ml penicillin, and 50 ?g/ml of streptomycin (Gibco) at 37?‚°C in 5-8% CO2. Medium is changed daily Inducible expression of Xist driven by a TetO promoter is achieved by adding DOX (1.5 ?g/ml).
Recommended controls: Xist-TetOP XY ESCs (Wutz et al., 2002 PMID: 11780141)
References:

Bousard et al. 2019. EMBO Rep. 20(10):e48019. PMID: 31456285.

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