#161901

TOV-2881EP

Cat. #161901

TOV-2881EP

Cat. #: 161901

Organism: Human

Tissue: Ovary

Disease: Cancer

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Anne-Marie Mes-Masson and Diane Provencher

Institute: Centre Hospitalier de L’université de Montréal

Primary Citation: Sauriol, et al. 2020. Cancers(Basel). 8,12(8):2222. PMID: 32784519

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: TOV-2881EP
  • Organism: Human
  • Tissue: Ovary
  • Donor: Patient was 56 at time of diagnosis wiht stage 3 IIIC ovarian high-grade serous adenocarcinoma with TP53 mutation. Donor had no prior cancer hisotry themselves, but had a malignant neoplasm of the urinary tract
  • Disease: Cancer
  • Morphology: Did not form spheroids
  • Growth properties: Mixed
  • Crispr: No
  • Description: Epithelial ovarian cancer cell line derived from solid tumors of patient who was diagnosted with Stage 3 IIIC ovarian high-grade serous adenocarcinoma had received neo-adjuvant chemotherapy of carboplatin/taxol prior to specimen collection in 2006. Cell line exhibited strong sensitivity to carboplatin. Cell line formed in vivo metastases and ascites when injected IP in mice, but did not form SC tumors. This cell line might be useful for studying disease spread at recurrence.
  • Production details: Tissue was scraped into 100mm plate with complete OSE medium and maintained for 40 days with weekly culture medium replacement
  • Biosafety level: 1

Handling

  • Format: Frozen
  • Growth medium: OSE medium, 10% FBS, 0.5ug/ml apmphotericin B and 50ug/ml gentamycin sulfate
  • Temperature: 37° C
  • Atmosphere: 7% O2 and 5% CO2
  • Shipping conditions: Dry Ice
  • Storage conditions: Liquid Nitrogen
  • Cultured in antibiotics: 2.5ug/ml amphotericin B and 50 ug/ml gentamicin
  • Characterisation tests: Whole exome sequencing, Gene expression microarray, IHC, Western Blot, etc.
  • Str profiling: TH01: 9 / D21S11: 32.2,33.2 / D5S818: 12 / D13S317: 9,11 / D7S820: 10,11 / D16S539: 12 / CSF1PO: 11 / vWA: 14,16 / TPOX: 11 / AMEL: X

References

  • Guo, et al. 2022. Front Genet. 25, 13:844800. PMID: 35401671
  • Sauriol, et al. 2020. Cancers(Basel). 8,12(8):2222. PMID: 32784519