#161936

SK-N-MC Cell Line

Cat. #161936

SK-N-MC Cell Line

Cat. #: 161936

Tissue: Brain

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: June L. Biedler, Barbara A. Spengler, Lawrence Helson

Institute: Memorial Sloan-Kettering Cancer Center (MSK)

Primary Citation: Biedler et al. Cancer research. 1973. 33(11):2643-2652. PMID: 4748425.

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: SK-N-MC Cell Line
  • Cancer: Neurological cancer
  • Cancers detailed: Neuroblastoma
  • Gender: Female
  • Tissue: Brain
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Products or characteristics of interest: Karyotype: The cell line is a pseudodiploid human female (XX), with chromosome counts in the diploid range and a modal chromosome number of 46. Normal chromosomes N3 and N10 are absent, and many (N1, N2, N4, N15, N16, N17, N21, and N22) are monosomic. Normal chromosome N8 is most often tetrasomic. The remainder of normal chromosomes were usually paired. Numerous marker chromosomes are present including: 1p+, der(3)t(2;3)(q24;q27), del(4)(p12), 11q+, del(2)(q23), ampl.(17)(p12), ampl.(16)(q13), del(15)(q13q22), 21p+, iso(3q), del(22)(q11q13). Marker chromosomes M2 and M3 appear to us to be identical to two markers (M4 and M3, respectively) described by R.C. Seeger, et al.for this cell line. Isoenzymes: AK-1, 1 ES-D, 2 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1 PGM3, 1-2
  • Description: SK-N-MC was originally described as a neuroblastoma cell line. It is now widely regarded as having originated from an Askin’s tumour (Ewing family of tumours).
  • Application: 3D cell culture, Neuroscience
  • Biosafety level: 1

Target Details

  • Target: Antigen expression: Blood Type O; Rh+

Applications

  • Application: 3D cell culture, Neuroscience

Handling

  • Growth medium: 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium then supplemented with fetal bovine serum to a final concentration of 10%.
  • Temperature: 37° C
  • Atmosphere: 5% CO2 in air
  • Shipping conditions: Dry Ice
  • Storage medium: Complete growth medium supplemented with 5% DMSO
  • Str profiling: Amelogenin: XCSF1PO: 10D13S317: 11D16S539: 12D5S818: 11D7S820: 8TH01: 9.3TPOX: 9,11vWA: 17,18D3S1358: 15D21S11: 30,31.2D18S51: 13,14Penta_E: 16,18Penta_D: 9,13D8S1179: 10FGA: 21,25D19S433: 13,15.2D2S1338: 17,21

References

  • Helson et al. 1975. Cancer Research. 35(9):2594-2599. PMID:167965.
  • Biedler et al. 1978. Cancer research. 38:3751-3757. PMID: 29704.