SK-LU-1 Cell Line

SK-LU-1 is a human lung adenocarcinoma cell line, primary that displays epithelial morphology and grows in adherent culture. This cell line expresses mutant K-Ras (G12D) and has homozygous deletions in the CDH6 and CDKN2A genes. These cells do not express the enzyme telomerase reverse transcriptase (hTERT) and consequently lack telomerase activity. This correlates with significantly […]

Contributors

Inventor Institute
Chester M. Southam Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161913
Cancer types: Lung cancer
Tissue: Lung
Gender: Female
Morphology: Epithelial
Growth properties: Adherent
Primary citation: Fogh J et al. 1977. Journal of the National Cancer Institute. 58: 209-214. PMID: 833871.
Product description: SK-LU-1 is a human lung adenocarcinoma cell line, primary that displays epithelial morphology and grows in adherent culture. This cell line expresses mutant K-Ras (G12D) and has homozygous deletions in the CDH6 and CDKN2A genes. These cells do not express the enzyme telomerase reverse transcriptase (hTERT) and consequently lack telomerase activity. This correlates with significantly reduced tumourigenicity in vitro and in vivo. These cells, however, display characteristics of alternative telomere lengthening (ALT) mechanisms (i.e., heterogeneity of telomere lengths and the presence of distinct nuclear structures called ALT-associated promyelocytic leukaemia bodies). The SK-LU-1 cells do not form tumours when injected into immunocompromised mice.
Gender: Female
Products or characteristics of interest: The stemline chromosome number is hypotetraploid, with the 2S component occurring at 4.4%. Marker chromosomes 1p, t(1q;11q); 11q+; t(13;?); 16q+; t(12q; 18q); M10; t(2q;13q); i(15); and ?t(xp;21q) occurred in all S metaphases, and t(1p;?); t(1p;14q); t(16;?), and t(14;21) occurred in some. In addition, 4 to 9 small markers of unidentifiable origin occurred frequently. Chromosome No. 7 was generally hexasomic, X chromosomes were disomic, and normal No. 15 was absent. No Y chromosome was detected in the QM stained preparation.
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry Ice
Growth medium: EMEM suplemented with 10% FBS.
Temperature: 37° C
Storage medium: Complete growth medium, 95%; DMSO, 5%
Recommended controls: –
Biosafety level: 1
References:

Fogh J et al. 1977. Journal of the National Cancer Institute. 58: 209-214. PMID: 833871.

Lehman et al. 1991. Cancer Research. 51: 4090-4096. PMID: 1855224.

Brachner A et al. 2006. Cancer Research. 66: 3584-3592. PMID: 16585183.

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