Cat. #161576

PaTu 8902 SMAD4 KO cell lines

Name: PaTu 8902 SMAD4 KO cell lines
SKU: 161576
Availability: InStock

Cat. #: 161576

Availability: 8-10 weeks

Organism: Human

Tissue: Pancreas

Disease: Cancer

Model: Knock out

£575.00

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Contributor

Inventor: Ji?? Neu?il

Institute: Institute of Biotechnology of the Academy of Sciences of the Czech Republic

Primary Citation: Ezrova et al. Oncogene. 2021 Apr, 40(14):2539-2552. PMID: 33686239

Tool Details

Handling

Related Tools

References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

Name: PaTu 8902 SMAD4 KO cell lines
Alternate name: PaTu 8902 SMAD4-deficient cells
Cancers detailed: Pancreatic ductal adenocarcinoma
Research fields: Cancer
Parental cell: PaTu 8902
Organism: Human
Gender: Female
Tissue: Pancreas
Disease: Cancer
Morphology: Epithelial cells
Growth properties: Adherent
Model: Knock out
Crispr: Yes
Conditional: No
Products or characteristics of interest: Crispr/Cas9 gene knock out
Description: SMAD4 is an important tumor suppressor involved in transforming growth factor ? (TGF?) signaling, and associated with altered mitochondrial activity. The resistance of SMAD4-deficient cells is mediated by increased mitophagic flux driven by MAPK/ERK signaling, whereas TGF?-induced resistance is autophagy-independent and linked to epithelial-to-mesenchymal transition (EMT). Mitochondria-targeted tamoxifen, a complex I inhibitor under clinical trial, overcomes resistance mediated by SMAD4-deficiency or TGF? signaling. Our data point to differential mechanisms underlying the resistance to treatment in PDAC arising from TGF? signaling and SMAD4 loss, respectively.
Biosafety level: 1
Recommended controls: PaTu 8902

Handling

Growth medium: DMEM+10% FBS
Temperature: 37° C
Atmosphere: 5% CO2
Shipping conditions: Dry Ice
Storage medium: Complete medium + 10% DMSO
Storage conditions: Liquid Nitrogen
Subculture routine: Trypsin-EDTA
Cultured in antibiotics: Yes
Mycoplasma free: Yes

Related Tools

Related tools: PaTu 8902 SMAD4 KOrec reconstituted cell line

References

Ezrova et al. Oncogene. 2021 Apr, 40(14):2539-2552. PMID: 33686239