#161788

OV-4485 cell line

Cat. #161788

OV-4485 cell line

Cat. #: 161788

Organism: Human

Tissue: Derived from ascites

£575.00

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Contributor

Inventor: Anne-Marie Mes-Masson and Diane Provencher

Institute: Centre Hospitalier de L’université de Montréal

Primary Citation: Fleury et al. 2015. Genes & Cancer. 6(9-10):378-398. PMID: 26622941

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: OV-4485 cell line
  • Alternate name: OV-4485
  • Organism: Human
  • Gender: Female
  • Tissue: Derived from ascites
  • Donor: Age of diagnosis: 55; BRCA hereditary status: BRCA1(+); Grade 2 – Stage IIIC; Mutations: TP53, BRCA1; Carboplatin/Taxol/maintenance Sorafenib vs Placebo trial
  • Morphology: Formed loosely compact spheroids with irregular margins
  • Growth properties: Adherent
  • Crispr: No
  • Receptors of note: No
  • Description: Epithelial ovarian cancer cell lines spontaneously derived from high-grade serious ascites. Patient harbored BRCA1:IVS14-1G>T mutation
  • Production details: Established from the cellular fraction of ascites collected by centrifugation. The cell lines were then maintained for 40 days in OSE with medium replaced weekly.
  • Additional notes: Patient 4485 was age 55 at diagnosis, BRCA hereditary status was BRCA1(+), and no prior personal history of cancer. Patient received Carboplatin/taxol, interval sugery, and was later in a clinical trial: maintenance sorafenib vs placebo trial.

Handling

  • Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
  • Atmosphere: Low oxygen conditions of 7% O2 and 5% CO2
  • Cultured in antibiotics: Amphotericin B and Gentamicin

References

  • Vias et al. 2023. Elife. 11
  • 12:e83867. PMID: 37166279 Sevinyan et al. 2022. Cancers (Basel). 16
  • 14(22):5628. PMID: 36428724 Asare-Werehene et al. 2023. Cancers (Basel). 30
  • 15(9):2566. PMID: 37174032