#161753

MSS-OM17 cell line

Cat. #161753

MSS-OM17 cell line

Cat. #: 161753

Organism: Human

Tissue: Omentum

Disease: Cancer

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Ziv Shulman, Irit Sagi, Roei Mazor

Institute: Weizmann Institute of Science

Primary Citation: Mazor et. al.. Cell, 2022. Mar 31,185(7):1208-1222. PMID: 35305314

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MSS-OM17 cell line
  • Alternate name: OM17
  • Organism: Human
  • Gender: Female
  • Tissue: Omentum
  • Disease: Cancer
  • Morphology: Rounded cells, large nuclei with prominent nucleoli
  • Growth properties: Adherent
  • Crispr: No
  • Products or characteristics of interest: p53 mutant, EpCAM(+)
  • Description: The cell line established directly from a tumor compartments (omental metastasis) of chemonaive HGSOC patient. Thus it closely recapitulates the tumor compartment as it emerged in these patients.
  • Application: Flow cytometry, Immunofluoresence staining and confocal microscopy
  • Production details: Fresh HGSOC omental metastases sample was retrieved from the operating theatre. Primary culture was established from this specimen as previously described (O Donnell et al. 2014) and used after 2-3 passages. Cell preparation included removing fibroblasts as well as all non-adherent cells from the culture. 100% of the cultured cells were EpCAM positive (324207, Biolegend, 1:200).
  • Biosafety level: 1
  • Recommended controls: Healthy fallopian tube epiuthelium

Applications

  • Application: Flow cytometry, Immunofluoresence staining and confocal microscopy

Handling

  • Volume: Frozen
  • Passage number: P2-3
  • Growth medium: DMEM media supplemented with 10% (v/v) foetal bovine serum, 1% (v/v) MEM-Eagle non essential amino acids, 1% (v/v) 2mM glutamine and 1% (v/v) Pen-Strep solution
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Shipping conditions: Dry ice
  • Storage medium: FBS + 10% (v/v) DMSO
  • Storage conditions: Liquid Nitrogen
  • Initial handling information: Thaw rapidly in preheated 37 °C growth media, centrifuge for 4 minutes in 300G, discard media, re-suspend and plate in preheated 37 °C growth media
  • Subculture routine: Rapidly growing cell line (doubeling time is roughly 24 hours). Trypsinize for 3-4 minutes in 37C until cells separate, add growth media (X4 volumes of trypsin). Split 1:4 - 1:10. Resuspend in growth media
  • Cultured in antibiotics: Penicillin / streptomycin
  • Characterisation tests: In flow cytometry, 100% of the cultured cells were EpCAM positive (324207, Biolegend, 1:200). See tab "EpCAM validation"

References

  • Mazor et. al.. Cell, 2022. Mar 31,185(7):1208-1222. PMID: 35305314