Melb-a cell line
Organism: Mouse
Tissue: Skin
Disease: Non-cancer
Model: Immortalised non-cancerous cell lines
£575.00
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Contributor
Inventor: Elena Sviderskaya
Institute: St. George's University London
Primary Citation: Sviderskaya EV, et al. Development. 1995 May;121(5):1547-57. PMID: 7540532
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Melb-a cell line
- Research fields: Tissue-specific biology
- Organism: Mouse
- Gender: Unspecified
- Tissue: Skin
- Disease: Non-cancer
- Morphology: Small, oval-fusiform
- Growth properties: Adherent
- Model: Immortalised non-cancerous cell lines
- Crispr: No
- Conditional: No
- Description: The melb-a cell line is an immortal melanoblast cell line derived from the skin of neonatal C57BL/6J (black, a/a) mice. These cells express dopachrome tautomerase (also referred to as tyrosinase-related protein 2) but generally do not express tyrosinase. These cells are unpigmented but can be induced to pigmented melanocytes if required (for example, as is the case for the melan-a2 cell line), also available from CancerTools.org. This melanoblast line is useful for developmental studies and, as melanoblasts display mechanisms similar to tumour cells (migration to and invasion of the epidermis) they are a useful tool in studies of melanoma development.
- Application: Melanocyte cell biology
- Biosafety level: BSL1
Applications
- Application: Melanocyte cell biology
Handling
- Growth medium: RPMI + FBS (10%) + fibroblast growth factor 2 (FGF2) (40 pM) + mouse stem cell factor (mSCF) (20 ng/ml)
- Storage conditions: liquid N2
References
- Sviderskaya EV, et al. Development. 1995 May
- 121(5):1547-57. PMID: 7540533
