Cat. #161910

Malme-3M Cell Line

Name: Malme-3M Cell Line
SKU: 161910
Availability: InStock

Cat. #: 161910

Availability: 8-10 weeks

Organism: Human

Tissue: Skin

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

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Contributor

Inventor: Jorgen Fogh, Germain Trempe

Institute: Memorial Sloan-Kettering Cancer Center (MSK)

Primary Citation: Fogh J et al. 1977. Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.

Tool Details

Target Details

Applications

Handling

References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

Name: Malme-3M Cell Line
Cancer type: Lung cancer
Cancers detailed: Malignant Melanoma
Organism: Human
Gender: Male
Tissue: Skin
Donor: 43-year-old, White male patient with malignant melanoma
Morphology: Mixed
Growth properties: Mixed: adherent and suspension
Description: Malme-3M is a malignant human melanoma cell line that displays fibroblast-like morphology and grows in mixed culture (adherent-suspension). This cell line has been shown to be dependent upon micropthalmia-associated transcription factor (MITF) activity for growth and survival. Malme-3M cells form tumours when injected into immunocompromised mice. These cells express mutant B-Raf (V600E) and wildtype N-Ras.
Application: 3D cell culture
Biosafety level: 1

Target Details

Target: Antigen expression: HLA A2, Aw30, B13, B40(+/-),DRw7

Applications

Application: 3D cell culture

Handling

Growth medium: Iscove's Modified Dulbecco's Medium supplemened with 20% FBS
Temperature: 37° C
Atmosphere: 95% Air, 5% CO2
Shipping conditions: Dry Ice
Storage medium: Culture medium 95%; DMSO, 5%
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Str profiling: Amelogenin: X,Y;CSF1PO: 12;D13S317: 8,13;D16S539: 9,12;D5S818: 11;D7S820: 9,12;TH01: 8;TPOX: 8,9;vWA: 15,16;D3S1358: 14,18;D21S11: 30.2,32.2;D18S51: 14;Penta_E: 11,12;Penta_D: 12,13;D8S1179: 13;FGA: 21,22;D19S433: 13,14;D2S1338: 24

References

Fogh J et al. 1977. Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.
Xing F et al. 2012. Oncogene. 31: 446-457. PMID: 21725359.
Ma J et al. 2013. PLoS One. 8: e73261. PMID: 24015299.