Cat. #161910
Malme-3M Cell Line
Cat. #: 161910
Availability: 8-10 weeks
Organism: Human
Tissue: Skin
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jorgen Fogh, Germain Trempe
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Fogh J et al. 1977. Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Malme-3M Cell Line
- Cancers detailed: Malignant Melanoma
- Organism: Human
- Gender: Male
- Tissue: Skin
- Donor: 43-year-old, White male patient with malignant melanoma
- Morphology: Mixed
- Growth properties: Mixed: adherent and suspension
- Description: Malme-3M is a malignant human melanoma cell line that displays fibroblast-like morphology and grows in mixed culture (adherent-suspension). This cell line has been shown to be dependent upon micropthalmia-associated transcription factor (MITF) activity for growth and survival. Malme-3M cells form tumours when injected into immunocompromised mice. These cells express mutant B-Raf (V600E) and wildtype N-Ras.
- Application: 3D cell culture
- Biosafety level: 1
Target Details
- Target: Antigen expression: HLA A2, Aw30, B13, B40(+/-),DRw7
Applications
- Application: 3D cell culture
Handling
- Growth medium: Iscove's Modified Dulbecco's Medium supplemened with 20% FBS
- Temperature: 37° C
- Atmosphere: 95% Air, 5% CO2
- Shipping conditions: Dry Ice
- Storage medium: Culture medium 95%; DMSO, 5%
- Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
- Str profiling: Amelogenin: X,Y;CSF1PO: 12;D13S317: 8,13;D16S539: 9,12;D5S818: 11;D7S820: 9,12;TH01: 8;TPOX: 8,9;vWA: 15,16;D3S1358: 14,18;D21S11: 30.2,32.2;D18S51: 14;Penta_E: 11,12;Penta_D: 12,13;D8S1179: 13;FGA: 21,22;D19S433: 13,14;D2S1338: 24
References
- Fogh J et al. 1977. Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.
- Xing F et al. 2012. Oncogene. 31: 446-457. PMID: 21725359.
- Ma J et al. 2013. PLoS One. 8: e73261. PMID: 24015299.
