KrasG12D.Smad4+/- murine lung cancer cell line

A novel syngeneic KrasG12D.Smad4+/- murine lung cancer cell line (X577) with orthotopic growth potential, that can serve as a useful preclinical model for evaluating tumour-host interactions, the impact of specific oncogenic alterations on the tumor microenvironment, and immunotherapeutic approaches. This cell line was derived from the primary lung tumour of a KrasLSL-G12D/+.Smad4fl/+ male C57BL/6 mouse […]

Contributors

Inventor Institute
Stephen Malkoski The University of Colorado Anschutz Medical Campus
Cat. #: 162384
Cancer types: Lung cancer
Research Fields: Cancer biology; Immunotherapy
Organism: Mouse
Tissue: Lung
Gender: Male
Primary citation: Nolan et al. 2020. Cancer Cell Int. 28: 20:417. PMID: 32874131
Alternate name: KrasG12D.Smad4+/- cell line; X577 cell line
Product description: A novel syngeneic KrasG12D.Smad4+/- murine lung cancer cell line (X577) with orthotopic growth potential, that can serve as a useful preclinical model for evaluating tumour-host interactions, the impact of specific oncogenic alterations on the tumor microenvironment, and immunotherapeutic approaches. This cell line was derived from the primary lung tumour of a KrasLSL-G12D/+.Smad4fl/+ male C57BL/6 mouse and is capable of forming orthotopic tumours in immunocompetent C57BL/6 mice. X577 cells demonstrate genetic recombination at the Kras and Smad4 loci as show by PCR, and express KRASG12D but not SMAD4, as shown by western analysis.
Gender: Male
Production details: A 6 week old KrasLSL-G12D/+.Smad4fl/+ male mouse was injected with 2 µl of 10^10 PFU/ml Ad5-CMV-Cre into the left lung. When this animal was euthanized 26 week later, a 12 mm primary tumor was passaged into a male recipient animal. After 3 passages through recipient animals (two of which included small lung tumors), the KrasG12D.Smad4+/- cell line (X577) was established that was capable of forming orthotopic tumours in immunocompetent C57BL/6 animals.
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Dulbecco's Modified Eagle Medium (DMEM) containing 4.5 g/l d-glucose, l-glutamine, and sodium pyruvate, supplemented with 10% (v/v) fetal bovine serum and 100 μg/ml of primocin (optional)
Subculture routine: Split 90% confluent cultures 1:5 to 1:15 as required. Cells are cultured at 37°C, in a humidified incubator with 5% CO2.
Temperature: 37°C
Atmosphere: 5% CO2
Biosafety level: 1
References:

Nolan et al. 2020. Cancer Cell Int. 28: 20:417. PMID: 32874131

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