Cat. #152756
CHO ICAM-1Fc Cell Line
Cat. #: 152756
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 3-5 days
Organism: Hamster
Tissue: Ovary
Model: Transgenic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Nancy Hogg
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: CHO ICAM-1Fc Cell Line
- Research fields: Cell biology;Immunology
- Tool sub type: Continuous
- Parental cell: Chinese Hamster Ovary (CHO)
- Organism: Hamster
- Tissue: Ovary
- Model: Transgenic
- Conditional: No
- Description: CHO ICAM-1Fc cells secrete a dimeric human ICAM-1Fc protein. ICAM-1 (CD54) is a major ligand for leukocyte CD11/CD18 integrins and used in adhesion assays to evaluate integrin activity.
- Production details: The chimeric wild-type ICAM-1Fc protein consists of the five extracellular domains of ICAM-1 joined to the hinge, CH2 and CH3 domains of IgG1. CHO cells were transfected using DEAE-dextran and the secreted proteins were collected in serum-free medium for 4 days. The proteins were purified on a Protein AÄË?Â?ÂSepharose affinity column using standard methods.
- Biosafety level: 1
- Recommended controls: Chinese Hamster Ovary (CHO)
- Cellosaurus id: CVCL_AS85
Target Details
- Target: Human ICAM-1Fc chimeric protein (five extracellular domains of ICAM-1 plus hinge, CH2 and CH3 domains of IgG1)
Handling
- Format: Frozen
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
References
- Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697.
- Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell.
- Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060.
- A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes.
- Smith et al. 2003. J Cell Sci. 116(Pt 15):3123-33. PMID: 12799414.
- LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent deta...
