Cat. #161632
1.4E7 cell line
Cat. #: 161632
Sub-type: Hybrid cell line
Organism: Human
Tissue: Pancreas
Disease: Diabetes
Model: Transgenic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Peter Flatt and Neville McClenaghan
Institute: Ulster University
Primary Citation: Lieber M et al. 1975. Int J Cancer 15: 741-747 PMID: 1140870
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: 1.4E7 cell line
- Research fields: Disease (not cancer)
- Tool sub type: Hybrid cell line
- Parental cell: PANC-1 cell line
- Organism: Human
- Tissue: Pancreas
- Disease: Diabetes
- Morphology: Epitheloid
- Growth properties: Adherent
- Model: Transgenic
- Model description: Hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line
- Crispr: No
- Description: A hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line. 1.4E7 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology.
- Application: producing pure insulin secreting cells when stimulated
- Additional notes: Patent: Flatt P & McClenaghan N (2004) Insulin producing cell-line US patent number 20040005702 (A1). Also WO0210346 (A2).
Applications
- Application: producing pure insulin secreting cells when stimulated
Handling
- Format: Frozen
- Growth medium: RPMI-1640 + 2mM Glutamine + 10% FCS
- Temperature: 37° C
- Atmosphere: 5% CO2
- Shipping conditions: Dry Ice
- Storage conditions: Liquid Nitrogen
- Initial handling information: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 104 cells/cm� using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 26hrs. At confluence 105 cells/cm� can be expected.
- Mycoplasma free: Yes
- Str profiling: STR-PCR Data:Amelogenin: XCSF1PO: 10,12D13S317: 11D16S539: 11D5S818: 11,13D7S820: 8THO1: 7,8TPOX: 8,11vWA: 15
References
- McCluskey JT et al. 2011. J Biol Chem. Apr 22. PMID: 21515691., Guo-Parke H et al. 2012. J Endocrinol. 214(3):257-65. PMID: 22685334
