Cat. #151303
Anti-CyclinA [E67.1]
Cat. #: 151303
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Cyclin A
Class: Monoclonal
Application: FACS ; IHC ; IF ; IP ; WB
Reactivity: Bovine ; Human ; Mink ; Mouse
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Julian Gannon
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CyclinA [E67.1]
- Alternate name: CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2Human Entrez Gene ID: 89 Human SwissProt: P2248 Human Unigene: 58974 Human Gene Symbol: CCNA2Human Chromosome Location: 4q27
- Research fields: Cancer;Cell biology;Genetics
- Clone: E67.1
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 54 kDa
- Reactivity: Bovine ; Human ; Mink ; Mouse
- Host: Mouse
- Application: FACS ; IHC ; IF ; IP ; WB
- Description: Monoclonal antibody capable of immunoprecipitating active CDK/Cyclin A H1 kinase.
- Immunogen: Full length bovine Cyclin A protein
- Immunogen uniprot id: P78396
- Isotype: IgG2a kappa
- Recommended controls: K-562 and HeLa Cells
Target Details
- Target: Cyclin A
- Molecular weight: 54 kDa
- Tissue cell line specificity: K-562 and HeLa Cells
- Target background: The anti-Cyclin A2, clone E67.1, recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located on amino acids 144-148 of human Cyclin A2. Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs), controlling transition at various specific phases of the cell cycle. Cyclin A is involved in the regulation of the cell cycle and is essential for progression through the S-phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S-phase, with passage through to the G2 phase. E67.1 is one of the only antibodies capable of immunoprecipitating active CDK/Cyclin A H1 kinase from such a wide range of species. This antibody binds to an epitope within amino acids 144-148 of human Cyclin A2, mapped out by phage selection.
Applications
- Application: FACS ; IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
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- Roilo et al. 2018. Nucleic Acids Res. 46(6):3198-3210. PMID: 29361038.
- Whalley et al. 2015. Nat Commun. 6:7437. PMID: 26078008.
- Ge et al. 2010. J Cell Biol. 191(7):1285-97. PMID: 21173116.
- Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories.
- Ho et al. 2008. J Biol Chem. 283(9):5477-85. PMID: 18077453.
- Identification of cyclin A2 as the downstream effector of the nuclear phosphatidylinositol 4,5-bisphosphate signaling network.
- Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
- Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.
- Sandhu et al. 2000. Oncogene. 19(47):5314-23. PMID: 11103932.
- Reduction of Cdc25A contributes to cyclin E1-Cdk2 inhibition at senescence in human mammary epithelial cells.
- Slingerland et al. 1994. Mol Cell Biol. 14(6):3683-94. PMID: 8196612.
- A novel inhibitor of cyclin-Cdk activity detected in transforming growth factor beta-arrested epithelial cells.
- Adamczewski et al. 1993. J Virol. 67(11):6551-7. PMID: 8411358.
- Simian virus 40 large T antigen associates with cyclin A and p33cdk2.