Anti-CyclinA [E67.1]

Cat. #151303

Anti-CyclinA [E67.1]

Cat. #: 151303

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Cyclin A

Class: Monoclonal

Application: FACS ; IHC ; IF ; IP ; WB

Reactivity: Bovine ; Human ; Mink ; Mouse

Host: Mouse


This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.


Inventor: Julian Gannon

Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details
Target Details

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-CyclinA [E67.1]
  • Alternate name: CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2Human Entrez Gene ID: 89 Human SwissProt: P2248 Human Unigene: 58974 Human Gene Symbol: CCNA2Human Chromosome Location: 4q27
  • Research fields: Cancer;Cell biology;Genetics
  • Clone: E67.1
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 54 kDa
  • Reactivity: Bovine ; Human ; Mink ; Mouse
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; IP ; WB
  • Description: Monoclonal antibody capable of immunoprecipitating active CDK/Cyclin A H1 kinase.
  • Immunogen: Full length bovine Cyclin A protein
  • Immunogen uniprot id: P78396
  • Isotype: IgG2a kappa
  • Recommended controls: K-562 and HeLa Cells

Target Details

  • Target: Cyclin A
  • Molecular weight: 54 kDa
  • Tissue cell line specificity: K-562 and HeLa Cells
  • Target background: The anti-Cyclin A2, clone E67.1, recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located on amino acids 144-148 of human Cyclin A2. Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs), controlling transition at various specific phases of the cell cycle. Cyclin A is involved in the regulation of the cell cycle and is essential for progression through the S-phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S-phase, with passage through to the G2 phase. E67.1 is one of the only antibodies capable of immunoprecipitating active CDK/Cyclin A H1 kinase from such a wide range of species. This antibody binds to an epitope within amino acids 144-148 of human Cyclin A2, mapped out by phage selection.


  • Application: FACS ; IHC ; IF ; IP ; WB


  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C


  • Jones et al. 2018. J Cell Biol. 217(9):3203-3218. PMID: 29930204.
  • Roilo et al. 2018. Nucleic Acids Res. 46(6):3198-3210. PMID: 29361038.
  • Whalley et al. 2015. Nat Commun. 6:7437. PMID: 26078008.
  • Ge et al. 2010. J Cell Biol. 191(7):1285-97. PMID: 21173116.
  • Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories.
  • Ho et al. 2008. J Biol Chem. 283(9):5477-85. PMID: 18077453.
  • Identification of cyclin A2 as the downstream effector of the nuclear phosphatidylinositol 4,5-bisphosphate signaling network.
  • Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
  • Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.
  • Sandhu et al. 2000. Oncogene. 19(47):5314-23. PMID: 11103932.
  • Reduction of Cdc25A contributes to cyclin E1-Cdk2 inhibition at senescence in human mammary epithelial cells.
  • Slingerland et al. 1994. Mol Cell Biol. 14(6):3683-94. PMID: 8196612.
  • A novel inhibitor of cyclin-Cdk activity detected in transforming growth factor beta-arrested epithelial cells.
  • Adamczewski et al. 1993. J Virol. 67(11):6551-7. PMID: 8411358.
  • Simian virus 40 large T antigen associates with cyclin A and p33cdk2.